Point-of-care
Quantitative Vitamin D Test
The test is an immunochromatography-based one step in vitro test. It is designed for the quantitative determination of total 25-hydroxy Vitamin D (25-OH Vitamin D) in human finger-prick blood. This assay provides a preliminary diagnostic test result and can be used for screening of Vitamin D deficiency. VHC Vitamin-D Quantitative Vitamin D Test utilizes the principle of immunochromatography, a unique two-site “Sandwich” immunoassay on a membrane. The test employs a very “Exclusive” pair of anti-25-OH Vitamin D Monoclonal Antibodies; one conjugated with colloidal gold and another one immobilized on the solid phase. This will selectively detect Vitamin D with a high degree of sensitivity and specificity. The liquid chromatography with tandem mass spectrometry (LC-MS/MS) assays or other quantitative immunoassays are recommended to further confirm the diagnostic test results.
Using a modern rapid test, such as the VHC Vitamin-D, a person’s current precise blood level can be determined on the spot. All that is required is a small drop of blood from the fingertip. This test provides prompt information as to whether and to which extent there is a vitamin D deficiency, and advice can be given there and then. The test result can be used to calculate the replenishment required to get up to a good vitamin D level. As the testing quantifies the deficit, the dosage to raise the level can be adjusted and reduced during the replenishment phase without risk of overdosing so that the optimum level for proper immune system activity is reached within a few weeks.
Perhaps more important
than ever before
Vitamin D and the immune system
It has been known for some years that the effects of vitamin D go far beyond optimum control of the calcium metabolism and the associated building and maintaining of healthy bones. In fact, it regulates the activity of over 1,000 genes in the human body. It has a key impact on the activity of many body functions, including the immune system.
The positive influence of an optimized vitamin D level on the immune system has been scientifically proven and convincingly substantiated by clinical trials. Consequently, drawing attention to this health-promoting characteristic by printing the phrase “Vitamin D supports normal immune function” on supplement packaging is permitted.
Bacterial and viral infections
Today, our modified lifestyle, involving activities independent of seasonal change, greater mobility, and regular new “external contacts”, is resulting in many people becoming more vulnerable to bacterial and viral infections with pathogens that are new to their immune system, particularly during the winter season.
The readiness and responsiveness of our entire immune system depend on an adequate vitamin D level in the blood. A recent study found that due to the fact that the body’s own production of vitamin D in the skin is often only ensured to a limited extent, some 80% of the German population are no longer building up a sufficient buffer to prevent a critical drop in the vitamin D level in winter.
Supplements may reduce risk
However, if they take a supplement during the winter season to keep their body’s vitamin D at an optimum level to maintain the responsiveness of the immune system, this minimizes their susceptibility to colds and the risk of contracting genuine flu (influenza). In clinical studies, scientists succeeded in reducing the general risk of contracting a respiratory disease by 35% through vitamin D supplementation.
Absorption of vitamin D via the digestive tract differs from person to person and depends on several factors. It, therefore, makes sense to check after 2–3 months which level has been achieved with the replenishment phase and the recommended maintenance dosage thereafter. Subsequently, the vitamin D level should be checked at least once a year to make sure that it is kept at a good level or to allow adjustment of the required dosage, if appropriate.
Performance characteristics
Sensitivity
The sensitivity of VHC Vitamin-D Quantitative Vitamin D Test is 3ng/ml (7.5nmol/l). The sensitivity was determined by calculating the mean plus 3.3 times of standard deviation of twenty Vitamin D-free serum tests.
Specificity
30 Vitamin D free serum samples were tested and all showed negative results; suggesting 100% specificity. No interference and cross reactivity was observed with Bilirubin, Triglycerides, Cholesterol, Vitamin B12 and Vitamin C.
Accuracy
Precision
| Sample | No of replicates | Mean ng/ml | Standard deviation | Coeficient Variation (CV) |
|---|---|---|---|---|
| Serum | 20 | 40.4 | 3.1 | 7.6% |
| Blood-1 | 10 | 33 | 2.5 | 7.6% |
| Blood-2 | 10 | 38.7 | 3.9 | 10.1% |
The complete package needed
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Start-up packages
- 10 x Tests and a Reader, ready to use
- 25 x Tests and a Reader, ready to use
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